Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names gap junction protein, alpha-like|gap junction 43 kDa heart protein|connexin 43|ODDD|GJAL|DFNB38|CX43|gap junction protein, alpha 1, 43kDa|GJA1 | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in HeLa and U2OS cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_000156.1 | |
Blocking Peptide EBP09301 | |
Immunogen Peptide with sequence C-QPFDFPDDNQNSKK, from the internal region of the protein sequence according to NP_000156.1. | |
Peptide Sequence C-QPFDFPDDNQNSKK | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Rat, Dog | |
Reactive Species Human | |
Human Gene ID 2697 | |
Rat Gene ID 24392 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:32000. | |
Western Blot Approx. 40kDa band observed in Human Cerebellum lysates (calculated MW of 43.0kDa according to NP_000156.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, IF, FC |
Goat Anti-Connexin 43 / GJA1 Antibody
$464.00
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Selected References [{"pmid": 26808661, "intro": "This antibody (previous batch) has been successfully used in IF on Rat:", "title": "Analysis of the behavioral, cellular and molecular characteristics of pain in severe rodent spinal cord injury.", "author": "Lee-Kubli CA, Ingves M, Henry KW, Shiao R, Collyer E, Tuszynski MH, Campana WM.", "journal": "Exp Neurol. 2016 Apr; 278:91-104."}] |