Goat Anti-Coronin 1 / TACO Antibody

$464.00

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SKU: EB06057
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
MGC117380|FLJ41407|coronin, actin-binding protein, 1A|coronin, actin-binding, 1A|coronin-1|coronin, actin binding protein, 1A|Clabp TACO|CLIPINA|HCORO1|CLABP|p57|TACO|Coronin 1|CORO1A
Usage Summary
Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
Accession ID
NP_009005.1
Blocking Peptide
EBP06057
Immunogen
Peptide with sequence C-KRLDRLEETVQAK, from the C Terminus of the protein sequence according to NP_009005.1.
Peptide Sequence
C-KRLDRLEETVQAK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human
Human Gene ID
11151
Mouse Gene ID
12721
Rat Gene ID
155151
Product Grade
IHC Results
Paraffin embedded Human Spleen and Small Intestine. Recommended concentration: 3.75µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx 60kDa band observed in lysates of cell lines Daudi, Jurkat and MOLT4 and approx. 55kDa in Human Lynph node and Spleen lysates (calculated MW of 51.0kDa according to Human NP_009005.1). These molecular weights are routinely observed by other sources. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, FC
Selected References
[{"pmid": 18544163, "intro": "This antibody (previous batch) has been successfully used in WB on Mouse:", "title": "Identification of differentially expressed proteins in spontaneous thymic lymphomas from knockout mice with deletion of p53.", "author": "Honoré B, Buus S, Claësson MH.", "journal": "Proteome Sci. 2008 Jun 10;6:18."}]