Goat Anti-CREB1 (aa96-109) Antibody

$423.00

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SKU: EB12171
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
transactivator protein|cyclic AMP-responsive element-binding protein 1|cAMP-responsive element-binding protein 1|cAMP-response element-binding protein-1|active transcription factor CREB|CREB-1|CREB|cAMP responsive element binding protein 1|CREB1
Usage Summary
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Accession ID
NP_004370.1; NP_604391.1
Blocking Peptide
EBP12171
Immunogen
Peptide with sequence C-DSQESVDSVTDSQK, from the internal region of the protein sequence according to NP_004370.1; NP_604391.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_004370.1; NP_604391.1).
Peptide Sequence
C-DSQESVDSVTDSQK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Dog, Pig, Cow
Reactive Species
Human, Mouse
Human Gene ID
1385
Mouse Gene ID
12912
Product Grade
IHC Results
Paraffin embedded Human Prostate. Recommended concentration: 2.5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 45kDa band observed in nuclear lysates of cell lines Jurkat and NIH3T3 (calculated MW of 36.7kDa according to NP_604391.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IHC
Selected References
[{"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}]