Goat Anti-CRTC2 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB10705
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
transducer of regulated cAMP response element-binding protein (CREB) 2| OTTMUSP00000023505| Torc2| 4632407F12Rik| CREB regulated transcription coactivator 2|Crtc2
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Accession ID
NP_083157.1
Blocking Peptide
EBP10705
Immunogen
Peptide with sequence C-DPAVEDSFRSDRLQ, from the C Terminus of the protein sequence according to NP_083157.1.
Peptide Sequence
C-DPAVEDSFRSDRLQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig, Cow
Reactive Species
Human, Rat
Human Gene ID
200186
Mouse Gene ID
74343
Rat Gene ID
310615
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx 90kDa observed in lysates of cell line Jurkat and in preliminary testing of NIH3T3, and approx 85kDa in Rat Brain lysates and lysates of cell line A431 (calculated MW of 73.2kDa according to Human, NP_083157.1) and Rat NP_001029067.1).These molecular weight s are routinely observed by other sources. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC

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