Goat Anti-Cryopirin / NALP3 / NLRP3 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB09884
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
NLRP3|NLR family, pyrin domain containing 3|NALP3|NACHT, LRR and PYD contain|NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3|MWS|FLJ95925|FCU|FCAS|CLR1.1|CIAS1|C1orf7|AVP|AII/AVP receptor-like|AII/AVP|AII|AGTAVPRL
Accession ID
NP_004886.3; NP_899632.1; NP_001120933.1; NP_001120934.1
Blocking Peptide
EBP09884
Immunogen
Peptide with sequence RDLYEKAKRDEPK-C, from the N-Terminus (near) of the protein sequence according to NP_004886.3; NP_899632.1; NP_001120933.1; NP_001120934.1.
Product Comments
This antibody is expected to recognize all reported isoforms (NP_004886.3; NP_899632.1; NP_001120933.1; NP_001120934.1). Reported variants NP_001073289.1 and NP_004886.3 represent identical protein.
Peptide Sequence
RDLYEKAKRDEPK-C
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
114548
Product Grade
IHC Results
Paraffin embedded Human peripheral blood leukocytes. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Preliminary experiments gave an approx 36kDa band in Human Peripheral Blood Mononucleocytes lysates and in lysates of cell line MOLT4, after 0.3-1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the smallest calculated size of 106kDa according to NP_899632.1. The 36kDa band was successfully blocked by incubation with the immunizing peptide.
Application Type
Pep-ELISA, IHC

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