Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names ferric-chelate reductase 3|FRRS3|duodenal cytochrome b|cytochrome b reductase 1|FLJ23462|DCYTB|CYBRD1 | |
Accession ID NP_079119.3 | |
Blocking Peptide EBP06633 | |
Immunogen Peptide with sequence CRNLALDEAGQRSTM, from the C Terminus of the protein sequence according to NP_079119.3. | |
Product Comments This antibody is expected to recognise isoform 1 (NP_079119.3) only. | |
Peptide Sequence CRNLALDEAGQRSTM | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Dog, Pig, Cow | |
Reactive Species Human | |
Human Gene ID 79901 | |
Product Grade  | |
IHC Results In paraffin embedded Human Testis shows membranous staining in primarily myofibroblasts of the basement membrane surrounding the seminiferous tubules. Recommended concentration: 2-5µg/ml. Paraffin embedded Human Small Intestine. Recommended concentration: 2.5ug/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:16000. | |
Western Blot Preliminary experiments gave bands at approx 35kDa and a 26+28kDa doublet in human colon lysate after 0.1µg/ml antibody staining. The detected bands were all successfully blocked by with the immunizing peptide. This antibody was successfully used in WB on Human in PMID: 24894955, 21973163 and 18830567. | |
Application Type Pep-ELISA, WB, IHC |
Goat Anti-Cytochrome b reductase 1 Antibody
$423.00
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Selected References [{"pmid": 18830567, "intro": "This antibody has been successfully used in WB on Human:", "title": "Differing expression of genes involved in non-transferrin iron transport across plasma membrane in various cell types under iron deficiency and excess.", "author": "Balusikova K, Neubauerova J, Dostalikova-Cimburova M, Horak J, Kovar J.", "journal": "Mol Cell Biochem. 2009 Jan;321(1-2):123-33."}, {"pmid": 24894955, "intro": "This antibody has been successfully used in WB on Human:", "title": "Role of duodenal iron transporters and hepcidin in patients with alcoholic liver disease.", "author": "Dostalikova-Cimburova M, Balusikova K, Kratka K, Chmelikova J, Hejda V, Hnanicek J, Neubauerova J, Vranova J, Kovar J, Horak J.", "journal": "J Cell Mol Med. 2014 Jun 3."}, {"pmid": 21973163, "intro": "This antibody has been successfully used in WB on Human:", "title": "Duodenal expression of iron transport molecules in patients with hereditary hemochromatosis or iron\r\ndeficiency.", "author": "Dostalikova-Cimburova M, Kratka K, Balusikova K, Chmelikova J, Hejda V, Hnanicek J, Neubauerova J, Vranova J, Kovar J, Horak J.", "journal": "J Cell Mol Med. 2012 Aug;16(8):1816-26."}] |