Goat Anti-DAG1 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

DAG1|dystroglycan 1 (dystrophin-associated glycoprotein 1)|dystroglycan 1|DAG|beta-dystroglycan|alpha-dystroglycan|AGRNR|A3a|156DAG

Immunofluorescence: Strong expression of the protein seen in the ER and cytoplasm of HeLa and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

NP_004384.5

EBP10102

Peptide with sequence C-HVGKHEYFMHATDK, from the internal region of the protein sequence according to NP_004384.5.

This antibody is expected to recognize both the precursor and the mature alpha-dystroglycan, but not the mature beta-dystroglycan.

C-HVGKHEYFMHATDK

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human

Human

1605

Paraffin embedded Human Heart. Recommended concentration: 5µg/ml.

Antibody detection limit dilution 1:128000.

Approx 100kDa band observed in Human Skeletal Muscle lysates (calculated MW of 97.4kDa according to Human NP_004384.4 ). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.

Pep-ELISA, WB, IHC, IF, FC