Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names DSP|DPI|desmoplakin I|desmoplakin|250kDa paraneoplastic pemphigus antigen | |
Accession ID NP_004406.2 | |
Blocking Peptide EBP10151 | |
Immunogen Peptide with sequence C-QESVKQAHLRNEH, from the internal region of the protein sequence according to NP_004406.2. | |
Product Comments This antibody is expected to recognize reported isoform I (NP_004406.2) only. | |
Peptide Sequence C-QESVKQAHLRNEH | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat | |
Reactive Species Human | |
Human Gene ID 1832 | |
Mouse Gene ID 109620 | |
Rat Gene ID 306871 | |
Product Grade  | |
IHC Results In paraffin embedded Human Heart shows staining of the junctons between cardiomuscular fibres. Recommended concentration, 3-6µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:4000. | |
Western Blot Preliminary experiments in lysates of cell lines HEK293, HeLa and NIH3T3 gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? | |
Application Type Pep-ELISA, IHC |
Goat Anti-Desmoplakin (aa 1672 -1684) Antibody
$423.00
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