Goat Anti-DKK1 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB06457
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
dickkopf related protein-1|dickkopf homolog 1|dickkopf (Xenopus laevis) homolog 1|dickkopf-1 like|dickkopf homolog 1 (Xenopus laevis)|DKK-1|SK|DKK1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HepG2 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_036374.1
Blocking Peptide
EBP06457
Immunogen
Peptide with sequence CDHHQASNSSRLHT, from the C Terminus of the protein sequence according to NP_036374.1.
Peptide Sequence
CDHHQASNSSRLHT
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Cow
Reactive Species
Human
Human Gene ID
22943
Product Grade
IHC Results
Paraffin embedded Human Placenta. Recommended concentration: 10µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 38kDa band observed in lysates of cell line MCF7 and in preliminary testing of HepG2 cell lysate and Human Spleen lysate (calculated MW of 28.7kDa accordin to NP_036374.1). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, IHC, FC

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