Goat Anti-DLC1 (Isoforms 1 and 3) Antibody

$423.00

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SKU: EB06436
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
deleted in liver cancer 1 variant 2|StAR-related lipid transfer (START) domain containing 12|StAR-related lipid transfer protein 12|START domain containing protein 12|Rho-GTPase-activating protein 7|deleted in liver cancer 1|p122-RhoGAP|FLJ21120|STARD12|ARHGAP7|HP|DLC1
Usage Summary
Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of U251 and U2OS cells. Recommended concentration: 10µg/ml.
Accession ID
NP_872584.2; NP_079043.3
Blocking Peptide
EBP06436
Immunogen
Peptide with sequence SVAIRKRSWEEHC, from the N Terminus of the protein sequence according to NP_872584.2; NP_079043.3.
Product Comments
This antibody is expected to recognise isoforms 1 and 3 ( NP_872584.2 and NP_079043.3 resp) but NOT isoform 2 (NP_006085.2).
Peptide Sequence
SVAIRKRSWEEHC
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Pig
Reactive Species
Human, Mouse
Human Gene ID
10395
Mouse Gene ID
50768
Product Grade
IHC Results
Paraffin embedded Mouse Adrenal Gland. Recommended concentration: 4µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Application Type
Pep-ELISA, IF, IHC
Selected References
[{"pmid": 19874489, "intro": "This antibody (previous batch) has been successfully used in WB in Human:", "title": "Deleted in liver cancer 1 isoforms are distinctly expressed in human tissues, functionally different and under differential transcriptional regulation in hepatocellular carcinoma.", "author": "Ko FC, Yeung YS, Wong CM, Chan LK, Poon RT, Ng IO, Yam JW.", "journal": "Liver Int. 2010 Jan;30(1):139-48."}]