Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names CSP-gamma|cysteine string protein-gamma|DNAJ (Hsp40) homolog, subfamily C, member 5 gamma|DNAJ homolog subfamily C member 5G|DNAJC5G|FLJ40417|gamma cysteine string protein|gamma-CSP|gamma-cysteine string protein | |
Accession ID NP_775921.1 | |
Blocking Peptide EBP11850 | |
Immunogen Peptide with sequence C-DSGRKYQQNVQSQP, from the internal region (near C Terminus) of the protein sequence according to NP_775921.1. | |
Peptide Sequence C-DSGRKYQQNVQSQP | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human | |
Reactive Species Human | |
Human Gene ID 285126 | |
Product Grade ![]() | |
IHC Results In paraffin embedded Human Testis shows nuclear staining primarily in spermatogonia and in cellular membrane. Recommended concentration, 5-10µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:128000. | |
Western Blot Preliminary experiments gave an approx 28kDa band in Human Testis lysates after 0.05µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 21.4kDa according to provisional NP_775921.1. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? | |
Application Type Pep-ELISA, IHC |
Goat Anti-DNAJC5G (human) Antibody
$423.00
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