Goat Anti-DNAJC6 Antibody

$255.00

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SKU: EB09621
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
auxilin|DnaJ (Hsp40) homolog, subfamily B, member 6|MGC48436|MGC129915|MGC129914|KIAA0473|DJC6|DnaJ (Hsp40) homolog, subfamily C, member 6|DNAJC6
Accession ID
NP_055602.1; NP_001243793.1; NP_001243794.1
Blocking Peptide
EBP09621
Immunogen
Peptide with sequence C-SPQNRPNYNVSFSA, from the internal region of the protein sequence according to NP_055602.1; NP_001243793.1; NP_001243794.1.
Peptide Sequence
C-SPQNRPNYNVSFSA
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog
Human Gene ID
9829
Mouse Gene ID
72685
Rat Gene ID
313409
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Preliminary experiments gave an approx 150kDa band in Mouse and Rat Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 100kDa according to Human NP_055602.1, 105kDa according to Mouse 001158055.1 and 99.3kDa according to Rat NP_001101419.1. The 150kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported
Application Type
Pep-ELISA