Goat Anti-DOT1L Antibody

$255.00

Category: Primary Antibodies
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SKU: EB06356
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
DOT1-like, histone H3 methyltransferase|histone methyltransferase DOT1L|KIAA1814|DOT1|DOT1-like, histone H3 methyltransferase (S. cerevisiae)|DOT1L
Usage Summary
Immunohistochemistry: Preliminary experiments in paraffin embedded Human kidney, shows cytoplasmic and nuclear staining of some epithelial cells in some renal tubules. Recommended concentration, 4µg/ml.
Accession ID
NP_115871.1
Blocking Peptide
EBP06356
Immunogen
Peptide with sequence C-SFSGVAGGTVGGN, from the C Terminus of the protein sequence according to NP_115871.1.
Peptide Sequence
C-SFSGVAGGTVGGN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse
Human Gene ID
84444
Mouse Gene ID
208266
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Western Blot: Preliminary experiments gave no signal but low background in Human Kidney and Testis lysates at up to 1µg/ml (Whole cell RIPA lysates not nuclear lysates). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Application Type
Pep-ELISA

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