Goat Anti-Doublecortex / DCX Antibody

$464.00

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SKU: EB07258
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
lissencephalin-X|doublin|doublecortin|OTTHUMP00000062892|XLIS|SCLH|LISX|DC|DBCN|RP5-914P14.1|X-linked (doublecortin)|lissencephaly|doublecortex|DCX
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 5µg/ml.
Accession ID
NP_000546.2; NP_835365.1; NP_835364.1; NP_835366.1
Blocking Peptide
EBP07258
Immunogen
Peptide with sequence C-KASPTPQKTSAKSPG, from the internal region of the protein sequence according to NP_000546.2; NP_835365.1; NP_835364.1; NP_835366.1.
Product Comments
This antibody is expected to recognise isoform a (NP_000546.2), isoform b (NP_835365.1), and isoform c (NP_835364.1 and NP_835366.1).
Peptide Sequence
C-KASPTPQKTSAKSPG
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human; Mouse, Rat, Dog, Cat
Reactive Species
Human, Mouse
Human Gene ID
1641
Product Grade
IHC Results
Paraffin embedded Human Brain (Cortex). Recommended concentration: 7µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 45kDa band observed in Mouse fetal Brain lysates (calculated MW of 40.6kDa according to Mouse NP_001103692.1). Recommended concentration: 0.003-0.01µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, IF