Goat Anti-DSS1 / SHFM1 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB06156
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
split hand/foot malformation type 1|deleted in split-hand/split-foot 1|deleted in split-hand/foot 1|candidate for split hand/foot malformation type 1|Shfdg1|SHSF1|SHFD1|SEM1|ECD|Deleted in split-hand/split-foot 1 region|DSS1|split hand/foot malformation (ectrodactyly) type 1|SHFM1
Usage Summary
Immunocytochemistry: Anonymous customer found nuclear staining in HT1080.
Accession ID
NP_006295.1
Blocking Peptide
EBP06156
Immunogen
Peptide with sequence C-RAELEKHGYKMETS, from the C Terminus of the protein sequence according to NP_006295.1.
Peptide Sequence
C-RAELEKHGYKMETS
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Dog, Cow, Pig
Human Gene ID
7979
Mouse Gene ID
20422
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
No signal obtained yet but a preliminary experiment showed low background in Human Liver extract at up to 1µg/ml. This used our standard western blotting protocol which we would not expect to detect proteins as small as the predicted size of approx. 8KDa according to NP_006295. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Application Type
Pep-ELISA, ICC

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