Goat Anti-DTX2 / Deltext-2 Antibody

$423.00

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SKU: EB09705
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
DTX2|deltex homolog 2 (Drosophila)|KIAA1528|MGC71098|RNF58|OTTHUMP00000024490|deltex 2|ring finger protein 58|zinc ion binding protein
Accession ID
NP_065943.2; NP_001096066.1
Blocking Peptide
EBP09705
Immunogen
Peptide with sequence C-EKLSTASGYSDVTD, from the internal region of the protein sequence according to NP_065943.2; NP_001096066.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_065943.2; NP_001096066.1). Reported variants NP_001096065.1, NP_065943.2, NP_001096064.1 represent identical protein.
Peptide Sequence
C-EKLSTASGYSDVTD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Rat, Cow
Reactive Species
Human
Human Gene ID
113878
Rat Gene ID
304591
Product Grade
IHC Results
Paraffin embedded Human Small Intestine. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Preliminary experiments gave an approx 85kDa band in lysates of cell lines A549, HepG2 and NIH3T3 after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.2kDa according to NP_065943.2. The 85kDa band was successfully blocked by incubation with the immunizing peptide.
Application Type
Pep-ELISA, IHC