Goat Anti-E2-230K / UBE2O Antibody

$423.00

Category: Primary Antibodies
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SKU: EB05761
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
KIAA1734|FLJ12878|E2-230K|ubiquitin-conjugating enzyme E2O|UBE2O
Accession ID
NP_071349.3
Blocking Peptide
EBP05761
Immunogen
Peptide with sequence ALLEAGMPECTEDK, from the C Terminus of the protein sequence according to NP_071349.3.
Peptide Sequence
ALLEAGMPECTEDK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
63893
Mouse Gene ID
217342
Product Grade
IHC Results
In paraffin embedded Human Heart shows textured staining in some myocardial fibres. Recommended concentration, 4-6µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Preliminary experiments in Human Colon lysates and in lysastes of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Application Type
Pep-ELISA, IHC

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