Goat Anti-EDF1 / MBF1 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB06304
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
multiprotein bridging factor 1|endothelial differentiation-related factor 1|MGC9058|EDF-1|MBF1|EDF1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_003783.1; NP_694880.1; NP_001268226.1; NP_001268227.1; NP_001268228.1
Blocking Peptide
EBP06304
Immunogen
Peptide with sequence AESDWDTVTVLRK-C, from the N Terminus of the protein sequence according to NP_003783.1; NP_694880.1; NP_001268226.1; NP_001268227.1; NP_001268228.1.
Peptide Sequence
AESDWDTVTVLRK-C
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat
Reactive Species
Human, Mouse
Human Gene ID
8721
Mouse Gene ID
59022
Product Grade
IHC Results
Paraffin embedded Human Pancreas and Brain (Cortex). Recommended concentration: 3.75µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 19kDa band observed in nuckear lysates of cell line HEK293, approx. 18kDa in nuckear lysates of cell line HeLa, and approx 17kDa in nuckear lysates of cell line NIH3T3 (calculated MW of 16.4kDa according to Human NP_003783.1 and Mouse NP_067494.1). Recommended concentration: 0.3-1µg/ml. This molecular weight is routinely observed by other sources. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC, IHC

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