Goat Anti-EHD2 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB06493
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
FLJ96617|EH domain containing 2|EH-domain containing 2|PAST2|EHD2
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the plasma membrane of A431 cells and the nuclei of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_055416.2
Blocking Peptide
EBP06493
Immunogen
Peptide with sequence CRLVPPSKRRHKGSA, from the C Terminus of the protein sequence according to NP_055416.2.
Product Comments
This antibody is expected to recognise EHD1 protein as well as EHD2.
Peptide Sequence
CRLVPPSKRRHKGSA
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human
Human Gene ID
30846
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx 70-75kDa band observed in Human Lung , Colon and Placenta lysates (calculated MW of 61.2kDa according to NP_055416.2). The observed molecular weight is observed by otherr sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC

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