Goat Anti-EIF2B4 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

EIF2B4|translation initiation facto|eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa|eukaryotic translation initiation factor 2B, subunit 4 delta|EIF2Bdelta|eIF-2B GDP-GTP exchange factor subunit delta|EIF-2B|EIF2B|DKFZp586J0119

NP_751945.2; NP_001029288.1; NP_056451.3

EBP10196

Peptide with sequence C-RKDYGSKVSLFSH, from the internal region of the protein sequence according to NP_751945.2; NP_001029288.1; NP_056451.3.

This antibody is expected to recognize all reported isoforms (NP_751945.2; NP_001029288.1; NP_056451.3).

C-RKDYGSKVSLFSH

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog, Cow

Human

8890

13667

117019

Antibody detection limit dilution 1:16000.

Approx 60kDa band observed in lysates of cell line K562 (calculated MW of 59.6kDa according to NP_751945.2). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA, WB