Goat Anti-ERG Antibody

$464.00

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SKU: EB07081
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
ets-related|TMPRSS2-ERG prostate cancer specific|OTTHUMP00000208036|OTTHUMP00000115970|v-ets erythroblastosis virus E26 oncogene homolog (avian)|v-ets erythroblastosis virus E26 oncogene like|v-ets avian erythroblastosis virus E26 oncogene related|transcriptional regulator ERG (transforming protein ERG)|p55|erg-3|HGNC:3446|v-ets erythroblastosis virus E26 oncogene like (avian)|ERG
Usage Summary
Immunofluorescence: Expression of the protein seen in the nucleus and cytoplasm of MCF7 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_891548.1; NP_004440.1; NP_001129626.1; NP_001129627.1; NP_001230358.1|
Blocking Peptide
EBP07081
Immunogen
Peptide with sequence C-PNTRLPTSHMPSH, from the C Terminus of the protein sequence according to NP_891548.1; NP_004440.1; NP_001129626.1; NP_001129627.1; NP_001230358.1|.
Product Comments
This antibody is expected to recognise all reported isoforms (NP_891548.1; NP_004440.1; NP_001129626.1; NP_001129627.1; NP_001230358.1)
Peptide Sequence
C-PNTRLPTSHMPSH
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
2078
Mouse Gene ID
13876
Rat Gene ID
170909
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx 55kDa band observed in lysates of cell line MOLT4 (calculated MW of 53.8kDa according to NP_891548.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, FC, IF