Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names retinal fascin|fascin 2|RP30|RFSN|fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) | |
Usage Summary Immunocytochemistry: This antibody has been successfully used in immunocytochemistry in Ling-Yun Zhou et al, Elife. 2023 Nov 20:12:e90155; PMID: 37982489. | |
Accession ID NP_001070650.1; NP_036550.1 | |
Blocking Peptide EBP08002 | |
Immunogen Peptide with sequence CHHRGSNQLDTNR, from the internal region of the protein sequence according to NP_001070650.1; NP_036550.1. | |
Product Comments This antibody is expected to recognize both reported isoforms (NP_001070650.1 and NP_036550.1). | |
Peptide Sequence CHHRGSNQLDTNR | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog | |
Reactive Species Mouse | |
Human Gene ID 25794 | |
Mouse Gene ID 238021 | |
Rat Gene ID 303741 | |
Product Grade  | |
IHC Results Paraffin embedded Mouse Brain. Recommended concentration: 4µg/ml. This product was also successfully used on sections of mouse cochlea as descibed in Shin et al, J Neurosci. 2010 Jul 21;30(29):9683-94; PMID: 20660251. | |
ELISA Detection Limit Antibody detection limit dilution 1:64000. | |
Application Type Pep-ELISA, IHC, ICC |
Goat Anti-Fascin 2 Antibody
$423.00
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Selected References [{"pmid": 22948144, "intro": "This antibody has been successfully used in the following paper:", "title": "HCN1 and HCN2 proteins are expressed in cochlear hair cells: HCN1 can form a ternary complex with protocadherin 15 CD3 and F-actin-binding filamin A or can interact with HCN2.", "author": "Ramakrishnan NA, Drescher MJ, Khan KM, Hatfield JS, Drescher DG.", "journal": "J Biol Chem. 2012 Nov 2;287(45):37628-46."}, {"pmid": 20660251, "intro": "This antibody has been successfully used in IHC on Mouse:", "title": "The R109H variant of fascin-2, a developmentally regulated actin crosslinker in hair-cell stereocilia, underlies early-onset hearing loss of DBA/2J mice.", "author": "Shin JB, Longo-Guess CM, Gagnon LH, Saylor KW, Dumont RA, Spinelli KJ, Pagana JM, Wilmarth PA, David LL, Gillespie PG, Johnson KR.", "journal": "J Neurosci. 2010 Jul 21;30(29):9683-94."}, {"pmid": 37982489, "intro": "This antibody has been successfully used in ICC on Mouse:", "title": "Differential regulation of hair cell actin cytoskeleton mediated by SRF and MRTFB.", "author": "Ling-Yun Zhou, Chen-Xi Jin, Wen-Xiao Wang, Lei Song, Jung-Bum Shin, Ting-Ting Du & Hao Wu", "journal": "Elife. 2023 Nov 20:12:e90155."}] |