Goat Anti-FEM1B Antibody

$255.00

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SKU: EB05043
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
FEM-1-like death receptor binding protein|FEM-1 (C.elegans) homolog b|KIAA0396|FIAA|fem-1 homolog b (C. elegans)|FEM1B
Usage Summary
Please note that the band we consistently detect in western blots differs from the predicted size of 69kDa. Currently we cannot find an explanation for this in the literature. However, splice prediction software (http://www.bioinformatics.ucla.edu/ASAP/) indicated the possibility of a shorter isoform. We would appreciate any feedback from people in the field - have any results been reported with other antibodies? Have any splice variants/modified forms been reported?
Accession ID
NP_056137
Blocking Peptide
EBP05043
Immunogen
Peptide with sequence CQIPRTLEEFVGFH, from the C Terminus of the protein sequence according to NP_056137.
Peptide Sequence
CQIPRTLEEFVGFH
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse
Reactive Species
Human
Human Gene ID
10116
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:2000.
Western Blot
Western blot: Approx 30-35kDa band seen in 293 cell lysates. Recommended for use at 1-3 µg/ml.