Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names 34 kDa nucleolar scleroderma antigen|34-kD nucleolar scleroderma antigen|FBL|FIB|fibrillarin|FLRN|RNA, U3 small nucleolar interacting protein 1|RNU3IP1|rRNA 2'-O-methyltransferase fibrillarin | |
Usage Summary Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. | |
Accession ID NP_001427.2 | |
Blocking Peptide EBP11641 | |
Immunogen Peptide with sequence CRGKEDALVTKN, from the internal region of the protein sequence according to NP_001427.2. | |
Peptide Sequence CRGKEDALVTKN | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Pig, Cow | |
Reactive Species Human | |
Human Gene ID 2091 | |
Mouse Gene ID 14113 | |
Rat Gene ID 292747 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:128000. | |
Western Blot Approx 35kDa band observed in lysates of cell line HEK293 (calculated MW of 33.8kDa according to NP_001427.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB |
Goat Anti-fibrillarin / FBL (aa99-110) Antibody
$423.00
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Selected References [{"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}] |