Goat Anti-Fubp1 (mouse, aa160-174) Antibody

$423.00

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SKU: EB11620
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
9530027K12Rik|D3Ertd330e|far upstream element (FUSE) binding protein 1|far upstream element (FUSE) binding protein 4|far upstream element-binding protein 1|FBP|Fubp|Fubp1|Fubp4|FUSE-binding protein 1
Usage Summary
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Accession ID
NP_476513.2
Blocking Peptide
EBP11620
Immunogen
Peptide with sequence C-DQIVEKGRPAPGFHH, from the internal region of the protein sequence according to NP_476513.2.
Peptide Sequence
C-DQIVEKGRPAPGFHH
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig, Cow
Reactive Species
Human
Human Gene ID
8880
Mouse Gene ID
51886
Rat Gene ID
654496
Product Grade
IHC Results
In paraffin embedded Human Tonsil shows staining in a selection of cells mostly outside the germinal centre. Recommended concentration: 4-8µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 75kDa band observed in lysates of cell line Jurkat (calculated MW of 67.4kDa according to NP_476513.2). Recommended concentration: 0.03-0.1µg/ml.
Application Type
Pep-ELISA, WB, IHC
Selected References
[{"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}]