Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names frizzled 8|frizzled (Drosophila) homolog 8|hFZ8|FZ-8|HGNC:4046|frizzled homolog 8 (Drosophila)|FZD8 | |
Usage Summary Immunofluorescence: This antibody has been successfully used in IF on Mouse: Zhao B et al. (2019) PMID: 21321602.
Additional validation: This antibody was successfully used in Electron microscopy (immunogold staining): Zhao B et al. (2019) PMID: 21321602. | |
Accession ID NP_114072.1 | |
Blocking Peptide EBP07028 | |
Immunogen Peptide with sequence C-SYPKQMPLSQV, from the C Terminus of the protein sequence according to NP_114072.1. | |
Peptide Sequence C-SYPKQMPLSQV | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Cow | |
Reactive Species Human | |
Human Gene ID 8325 | |
Mouse Gene ID 14370 | |
Product Grade  | |
IHC Results Paraffin embedded Human Pancreas. Recommended concentration: 3µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:32000. | |
Western Blot Approx 75kDa band observed in Human Pancreas lysates (calculated MW of 73.3kDa according to NP_114072.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Mouse: Zhao B et al. (2019) PMID: 21321602. | |
Application Type Pep-ELISA, WB, IHC, IF, EM |
Goat Anti-FZD8 / frizzled 8 Antibody
$464.00
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Selected References [{"pmid": 21321602, "intro": "This antibody has been successfully used in WB, IF & EM on Mouse:", "title": "Transport of receptors, receptor signaling complexes and ion channels via neuropeptide-secretoryvesicles.", "author": "Zhao B, Wang HB, Lu YJ, Hu JW, Bao L, Zhang X.", "journal": "Cell Res. 2011 May;21(5):741-53."}] |