Goat Anti-GATA1 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

erythroid transcription factor 1|NF-E1 DNA-binding protein|XLTT|GF-1|transcription factor GATA1|globin transcription factor 1|erythrold transcription factor 1|GATA-binding protein 1 (globin transcription factor 1)|GATA binding protein 1|NFE1|GF1|ERYF1|HGNC:4170|GATA binding protein 1 (globin transcription factor 1)|GATA1

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and NIH3T3 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

NP_002040.1

EBP06874

Peptide with sequence C-DAEAYRHSPVFQ, from the internal region of the protein sequence according to NP_002040.1.

C-DAEAYRHSPVFQ

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog, Cow

Human, Mouse

2623

Antibody detection limit dilution 1:16000.

Approx 50-55kDa band observed in nuclear lysates of cell line K562 calculated MW of 42.8kDa according to NP_002040.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Hippocampus lysate.

Pep-ELISA, WB, IF, FC