Goat Anti-GPR94 / TRA1 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB07290
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
tumor rejection antigen (gp96) 1|glucose regulated protein, 94 kDa|endothelial cell (HBMEC) glycoprotein|Tumor rejection antigen-1 (gp96)|GP96|ECGP|HGNC:12028|tumor rejection antigen (gp96) 1|GRP94|TRA1
Usage Summary
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Accession ID
NP_003290.1
Blocking Peptide
EBP07290
Immunogen
Peptide with sequence C-KEGVKFDESEKTKE, from the internal region of the protein sequence according to NP_003290.1.
Peptide Sequence
C-KEGVKFDESEKTKE
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig, Cow, Zebrafish
Reactive Species
Human
Human Gene ID
7184
Mouse Gene ID
22027
Rat Gene ID
362862
Product Grade
IHC Results
Paraffin embedded Human Liver. Recommended concentration: 5-7µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:8000.
Western Blot
Approx. 100kDa band observed in lysates of cell lines HeLa, HepG2 and U2OS and in preliminary testing of NIH3T3 cell lysate and Mouse Liver lysate (calculated MW of 92.5kDa according to Human NP_003290.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-0.5µg/ml. Primary incubation 1 hour at room temperature. Positive Control: A batch specific positive control lysate is available for this product. Please contact [email protected] for availability.
Application Type
Pep-ELISA, WB, IHC, FC

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