Goat Anti-Hamartin / TSC1 (isoform 1) Antibody

$423.00

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SKU: EB07221
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
tumor suppressor|tuberous sclerosis 1 protein|TSC|MGC86987|LAM|KIAA0243|HGNC:12362|tuberous sclerosis 1|hamartin|TSC1
Accession ID
NP_000359.1
Blocking Peptide
EBP07221
Immunogen
Peptide with sequence C-QLHIMDYNETHHEH, from the C Terminus of the protein sequence according to NP_000359.1.
Product Comments
This antibody is expected to recognise isoform 1 (NP_000359.1) only.
Peptide Sequence
C-QLHIMDYNETHHEH
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog
Reactive Species
Human
Human Gene ID
7248
Mouse Gene ID
64930
Product Grade
IHC Results
Paraffin embedded Human Uterus and Liver. Recommended concentration: 3.75µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 150kDa band observed in Human Brain lysates (calculated MW of 130kDa according to NP_000359.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miloloza et al Hum Mol Genet. 2000 Jul 22;9(12):1721-7; PMID: 10915759). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IHC