Goat Anti-HAP1 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB07528
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
neuroan 1|huntingtin-associated protein 2|huntingtin-associated protein 1|hHLP1|HLP|HIP5|HAP2|huntingtin-associated protein 1 (neuroan 1)|HAP1
Accession ID
NP_003940.2; NP_817084.1
Blocking Peptide
EBP07528
Immunogen
Peptide with sequence C-RYDFRYSEDREQ, from the internal region of the protein sequence according to NP_003940.2; NP_817084.1.
Product Comments
This antibody is expected to recognize all reported isoforms (NP_003940.1, NP_817084.1)
Peptide Sequence
C-RYDFRYSEDREQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
9001
Product Grade
IHC Results
Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 70kDa band observed in Human Brain (Frontal Cortex and Hippocampus) lysates (calculated MW of 69.6kDa according to NP_817084.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 25kDa. This band was successfully blocked by incubation with the immunising peptide.
Application Type
Pep-ELISA, WB, IHC

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