Goat Anti-HEM1 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

membrane-associated protein hem-1|hematopoietic protein 1|HEM1|NCK-associated protein 1-like|NCKAP1L

NP_005328.2; NP_001171905.1

EBP08890

Peptide with sequence C-RHTENVTKTKTPE, from the internal region of the protein sequence according to NP_005328.2; NP_001171905.1.

C-RHTENVTKTKTPE

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Cow

3071

105855

315348

Antibody detection limit dilution 1:16000.

Preliminary experiments gave an approx. 50kDa band in Human Tonsil and Thymus lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 128kDa according to NP_005328.2. The 50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA