Goat Anti-HEXIM1 Antibody

$423.00

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SKU: EB06964
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
FLJ13562|EDG1|menage a quatre 1|hexamthylene bis-acetamide inducible 1|hexamethylene-bis-acetamide-inducible transcript 1|hexamethylene bisacetamide-inducible protein|HMBA-inducible|MAQ1|HIS1|CLP1|HGNC:24953|hexamethylene bis-acetamide inducible 1|HEXIM1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nucleus of A431 and HeLa cells. Recommended concentration: 10µg/ml.
Accession ID
NP_006451.1
Blocking Peptide
EBP06964
Immunogen
Peptide with sequence C-HRQQERAPLSKFGD, from the C Terminus of the protein sequence according to NP_006451.1.
Peptide Sequence
C-HRQQERAPLSKFGD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse
Reactive Species
Human, Mouse
Human Gene ID
10614
Mouse Gene ID
192231
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 40kDa band observed in Human and Mouse Heart lysates (calculated MW of 40.6kDa according to Human NP_006451.1 and 40.2kDa according to Mouse NP_620092.1. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF
Selected References
[{"pmid": 0, "intro": "This antibody has been successfully used in the following paper:", "title": "Inhibition of CDK12 elevates cancer cell dependence on P-TEFb by stimulation of RNA polymerase II pause release", "author": "Zhijia Wang, Samu V. Himanen, Heidi M. Haikala, Caroline C. Friedel, Anniina Vihervaara, Matjaž Barbori?", "journal": "(2023) https://doi.org/10.1101/2023.03.21.533608"}, {"pmid": 30824372, "intro": "This antibody (previous batch) has been successfully used in IP on Human:", "title": "P-TEFb activation by RBM7 shapes a pro-survival transcriptional response to genotoxic stress", "author": "Andrii Bugai, Alexandre J.C. Quaresma, Caroline C. Friedel, Tina Lenasi, Christopher R. Sibley, Petra Kukanja, Koh Fujinaga, Melanie Blasius, Thomas Hennig, Jernej Ule, Lars Dölken, Matjaz Barboric", "journal": "Mol Cell. 2019 Apr 18;74(2):254-267.e10."}, {"pmid": 26499373, "intro": "This antibody (previous batch) has been successfully used in Western blot on Human:", "title": "The CD8+ cell non-cytotoxic antiviral response affects RNA polymerase II-mediated human immunodeficiency virus transcription in infected CD4+ cells.", "author": "Dalibor Blazek, Fernando Teque, Carl Mackewicz, Matija Peterlin and Jay A. Levy.", "journal": "J Gen Virol. 2016 Jan;97(1):220-4."}, {"pmid": 38301887, "intro": "This antibody has been successfully used in Western blot on Human:", "title": "Actin associates with actively elongating genes and binds directly to the Cdk9 subunit of P-TEFb.", "author": "Salla Kyheröinen et al.", "journal": "J Biol Chem. 2024 Mar;300(3):105698."}]