Goat Anti-HIF1alpha / MOP1 Antibody

$423.00

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SKU: EB10789
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
PAS domain-containing | OTTHUMP00000179063| HIF-1-alpha| ARNT interacting protein| bHLHe78| PASD8| MOP1| HIF1-ALPHA| HIF1| HIF-1alpha| hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)|HIF1A
Accession ID
NP_001521.1; NP_851397.1
Blocking Peptide
EBP10789
Immunogen
Peptide with sequence C-KMTQLFTKVESED, from the internal region of the protein sequence according to NP_001521.1; NP_851397.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_001521.1; NP_851397.1). This antibody is NOT expected to cross-react with EPAS1 (GeneID 2034).
Peptide Sequence
C-KMTQLFTKVESED
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human. Mouse, Rat, Dog, Pig, Cow
Reactive Species
Human, Mouse
Human Gene ID
3091
Mouse Gene ID
15251
Rat Gene ID
29560
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:1000.
Western Blot
Approx 90kDa band observed in Human and Mouse Lung and in Human Kidney lysates and approx 85-90kDa in Human Gastrointestinal cancer lysates (calculated MW of 92.7kDa according to Human NP_001521.1, 82.7kDa according to Human NP_851397.1 and 93.7kDa according to Mouse NP_001300848.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB