Goat Anti-HNF4A Antibody

$423.00

Category: Primary Antibodies
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SKU: EB06700
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
HNF4alpha10/11/12|MODY1|MODY|HNF4alpha|FLJ39654|transcription factor-14|HNF4-alpha|hepatocyte nuclear factor 4, alpha|NR2A21|HNF4a9|HNF4a8|HNF4a7|TCF14|NR2A1|HNF4|TCF|HNF4A
Accession ID
NP_849180.1; NP_000448.3; NP_849181.1
Blocking Peptide
EBP06700
Immunogen
Peptide with sequence RLSKTLVDMDMADY-C, from the N Terminus of the protein sequence according to NP_849180.1; NP_000448.3; NP_849181.1.
Product Comments
This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1; NP_000448.3; NP_849181.1 resp.).
Peptide Sequence
RLSKTLVDMDMADY-C
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Dog, Pig
Reactive Species
Human
Human Gene ID
3172
Product Grade
IHC Results
Paraffin embedded Human Small Intestine. Recommended concentration: 2µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 55kDa and 48kDa bands observed in lysates of cell line HepG2 (calculated MW of 52.8kDa according to Human NP_000448.3 , 46.6kDa according to Human NP_849181.1). Recommended concentration: 0.1-0.5µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IHC

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