Goat Anti-HNF4A Antibody

$423.00

Category: Primary Antibodies
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SKU: EB07649
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
transcription factor-14|hepatocyte nuclear factor 4 alpha|hepatic nuclear factor 4 alpha|HNF4-alpha|TCF14|TCF|NR2A21|NR2A1|MODY1|MODY|HNF4a9|HNF4a8|HNF4a7|HNF4|FLJ39654|hepatocyte nuclear factor 4, alpha|HNF4A
Usage Summary
Immunofluorescence: Expression of the protein seen in the nucleus of NIH3T3 cells. Recommended concentration: 10µg/ml.
Accession ID
NP_849180.1; NP_000448.3; NP_787110.2; NP_001025174.1; NP_001245284.1; NP_001274111.1; NP_001274112.1
Blocking Peptide
EBP07649
Immunogen
Peptide with sequence C-HPHLMQEHMGTN, from the internal region of the protein sequence according to NP_849180.1; NP_000448.3; NP_787110.2; NP_001025174.1; NP_001245284.1; NP_001274111.1; NP_001274112.1.
Product Comments
This antibody is expected to recognise isoforms 1, 2, 4, 5, 7, 8 and 9
Peptide Sequence
C-HPHLMQEHMGTN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog
Reactive Species
Mouse
Human Gene ID
3172
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Preliminary experiments showed an approx 55kDa band in Human Colon, Duodenum, Kidney and Liver lysates (calculated MW of 52.8kDa according to NP_000448.3). Recommended concentration:0.3-1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, IF

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