Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names YDR533C|Hsp31p | |
Accession ID NP_010822.1 | |
Blocking Peptide EBP11539 | |
Immunogen Peptide with sequence C-ETGKFGWDEHSLAKD, from the internal region of the protein sequence according to NP_010822.1. | |
Peptide Sequence C-ETGKFGWDEHSLAKD | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Saccharomyces cerevisiae S288c | |
Reactive Species S. cerevisiae | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:128000. | |
Western Blot Approx 24kDa band observed in lysates of postdiauxic (overnight grown yeast cells, and no signal in KO grown under identical conditions. Cells grown in logarithmic phase show diminished signals as expected. (calculated MW of 25.7kDa according to NP_010822.1). Recommended concentration: 0.02-0.06µg/ml. This antibody has been successfully used in the following paper: Natka?ska et al (2018) PMID: 29264711. | |
Application Type Pep-ELISA, WB |
Goat Anti-Hsp31p (yeast, aa50-64) Antibody
$423.00
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Selected References [{"pmid": 29264711, "intro": "This antibody has been successfully used in Redox WB on Yeast:", "title": "Oxidative stress triggers aggregation of GFP-tagged Hsp31p, the budding yeast environmental stress response chaperone, and glyoxalase III", "author": "Urszula Natka?ska, Adrianna Skoneczna and Marek Skoneczny", "journal": "Cell Stress and Chaperones (2018) 23:595–607"}] |