Goat Anti-HSP60 / HSPD1 (aa333-344) Antibody

$464.00

Category: Primary Antibodies
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SKU: EB12834
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
mitochondrial matrix prote|heat shock protein 65|chaperonin 60|P60 lymphocyte protein|60 kDa heat shock protein, mitochondrial|60 kDa chaperonin|SPG13|HuCHA60|HSP65|HSP60|HSP-60|HLD4|GROEL|CPN60|heat shock 60kDa protein 1 (chaperonin)|HSPD1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the mitochondria of HeLa cells and in the cytoplasm of A431 cells. Recommended concentration: 10µg/ml.
Accession ID
NP_002147.2
Blocking Peptide
EBP12834
Immunogen
Peptide with sequence C-NLEDVQPHDLGK, from the internal region of the protein sequence according to NP_002147.2.
Product Comments
Reported variants represent identical protein: NP_955472.1, NP_002147.2.
Peptide Sequence
C-NLEDVQPHDLGK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Dog, Pig, Cow
Reactive Species
Human, Rat, Pig
Human Gene ID
3329
Product Grade
IHC Results
Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx 60kDa band observed in Human and Pig Heart, Kidney and Liver and in Rat Kidney lysates (calculated MW of 61.1kDa according to Human NP_002147.2 and 60.9kDa according to Pig NP_001241645.1 and Rat NP_071565.2 ). Recommended concentration: 0.01-0.1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, IF

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