Goat Anti-HSPC150 / UBE2T Antibody

$464.00

Category: Primary Antibodies
SKU Unit Size Price

Select a unit size:

SKU: EB05765
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
ubiquitin-conjugating enzyme E2T|ubiquitin-conjugating enzyme E2T (putative)|ubiquitin conjugating enzyme|HSPC150 protein similar to ubiquitin-conjugating enzyme|PIG50|HSPC150|UBE2T
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Accession ID
NP_054895.1; NP_001297255.1
Blocking Peptide
EBP05765
Immunogen
Peptide with sequence C-QLVGIEKKFHPDV, from the C Terminus of the protein sequence according to NP_054895.1; NP_001297255.1.
Peptide Sequence
C-QLVGIEKKFHPDV
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
29089
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx. 23-24kDa band observed in nuclear lysates of cell lines HeLa, Jurkat, HEK293, HepG2 and in THP-1 cell lysates, and approx. 25kDa in lnuclear lysates of cell line A431 and in K562, cell lysatess (calculated MW of 22.5kDa according to NP_054895.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC

Related products