Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names HTATSF1|HIV-1 Tat specific factor 1|RP1-196E23.2|TAT-SF1|dJ196E23.2|HIV TAT specific factor 1|cofactor required for Tat activation of HIV-1 transcription | |
Usage Summary Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. | |
Accession ID NP_055315.2 | |
Blocking Peptide EBP07999 | |
Immunogen Peptide with sequence C-QELYGDGKDGDTQTD, from the internal region (near the N Terminus) of the protein sequence according to NP_055315.2. | |
Peptide Sequence C-QELYGDGKDGDTQTD | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Rat, Dog | |
Reactive Species Human | |
Human Gene ID 27336 | |
Rat Gene ID 317612 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:16000. | |
Western Blot Approx 140kDa band observed in lysates of cell line Daudi (calculated MW of 85.9kDa according to NP_055315.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhou and Sharp, Science. 1996 Oct 25;274(5287):605-10.; PMID: 8849451). Recommended concentration: 1-3µg/ml.
An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunising peptide. | |
Application Type Pep-ELISA, WB |
Goat Anti-HTATSF1 Antibody
$423.00
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Selected References [{"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}] |