Goat Anti-HTR1A (aa249-262) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

HTR1A| 5-hydroxytryptamine (serotonin) receptor 1A| 5-HT1A| 5HT1a| ADRB2RL1| ADRBRL1| 5-HT-1A| 5-HT1a receptor| 5-hydroxytryptamine receptor 1A| G protein coupled receptor| G-21| OTTHUMP00000221821| guanine nucleotide-binding regulatory protein-coupled receptor| serotonin receptor 1A

NP_000515.2

EBP11005

Peptide with sequence C-QPKKSVNGESGSRN, from the internal region of the protein sequence according to NP_000515.2.

C-QPKKSVNGESGSRN

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human

3350

Antibody detection limit dilution 1:16000.

Preliminary experiments gave bands at approx 37kDa and 35kDa in Human Brain (Hippocampus, Substantia nigra) lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 46.1kDa according to NP_000515.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA