Goat Anti-IER2 / ETR101 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

T-lymphocyte activated protein|Pip92|immediate early protein|ETR101|HGNC:28871|immediate early response 2|IER2

Immunohistochemistry: Preliminary experiments in paraffin embedded Human Tonsil showed staining in follicles, interfollicular area and endothelial cells. Recommended concentration 0.1-0.3ug/ml

NP_004898.2

EBP07022

Peptide with sequence EVQKEAQRIMTL-C, from the N Terminus of the protein sequence according to NP_004898.2.

EVQKEAQRIMTL-C

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog

9592

Antibody detection limit dilution 1:128000.

Western Blot: Preliminary experiments gave an approx 60kDa band in Human Brain lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 24.2kDa according to NP_004898.2. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA