Goat Anti-IL18 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB09393
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
interleukin-18|interleukin-1 gamma|interleukin 18|interferon-gamma-inducing factor|IL-1 gamma|MGC12320|IL1F4|IL-1g|IL-18|IGIF|interleukin 18 (interferon-gamma-inducing factor)|IL18
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the golgi and cytoplasm of A431and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_001553.1; NP_001230140.1
Blocking Peptide
EBP09393
Immunogen
Peptide with sequence C-NPPDNIKDTKSDI, from the internal region of the protein sequence according to NP_001553.1; NP_001230140.1.
Product Comments
This antibody is expected to recognise both reported isoforms (NP_001553.1; NP_001230140.1).
Peptide Sequence
C-NPPDNIKDTKSDI
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
3606
Rat Gene ID
29197
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 23kDa band observed in lysates of cell line HeLa and approx. 21-22kDa band in lysates of cell lines A431 and U2OS (calculated MW of 22.3kDa according to NP_001553.1). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC

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