Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names nuclear factor of activated T-cells, 45-kDa|interleukin enhancer binding factor 2|PRO3063|MGC8391|interleukin enhancer binding factor 2, 45kDa|NF45|ILF2 | |
Accession ID NP_004506.2; NP_001254738.1 | |
Blocking Peptide EBP07784 | |
Immunogen Peptide with sequence C-EKAYEKPPEKKEG, from the internal region (near the C Terminus) of the protein sequence according to NP_004506.2; NP_001254738.1. | |
Product Comments This antibody is expected to recognize both reported isoforms (NP_004506.2; NP_001254738.1). | |
Peptide Sequence C-EKAYEKPPEKKEG | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog | |
Reactive Species Human | |
Human Gene ID 3608 | |
Mouse Gene ID 67781 | |
Rat Gene ID 310612 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:4000. | |
Western Blot Approx 43kDa band observed in Human Tonsil and Thymus lysates (calculated MW of 43.1kDa according to NP_004506.2). Recommended concentration: 0.03-0.1µg/ml. This antibody has been successfully used in WB in the following papers: Ye J et al, RNA. 2017 Aug;23(8):1270-1284. PMID: 28487382. And Shamanna RA et al, Oncogene. 2013 Oct 24;32(43):5176-85 PMID: 23208500. | |
Application Type Pep-ELISA, WB |
Goat Anti-ILF2 / NF45 Antibody
$423.00
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Selected References [{"pmid": 21969602, "intro": "This antibody (previous batch) has been successfully used in WB and IP on HeLa cells:", "title": "The NF90/NF45 complex participates in DNA break repair via nonhomologous end joining.", "author": "Shamanna RA, Hoque M, Lewis-Antes A, Azzam EI, Lagunoff D, Pe'ery T, Mathews MB.", "journal": "Mol Cell Biol. 2011 Dec;31(23):4832-43."}, {"pmid": 28487382, "intro": "This antibody has been successfully used in WB and IP on ESCs:", "title": "NF45 and NF90/NF110 coordinately regulate ESC pluripotency and differentiation", "author": "Ye J, Jin H, Pankov A, Song J, Blelloch R.", "journal": "RNA. 2017 Aug;23(8):1270-1284. "}, {"pmid": 23208500, "intro": "This antibody has been successfully used in WB on Human:", "title": "Induction of p53, p21 and apoptosis by silencing the NF90/NF45 complex in human papilloma virus-transformed cervical carcinoma cells.", "author": "Shamanna RA, Hoque M, Pe'ery T, Mathews MB.", "journal": "Oncogene. 2013 Oct 24;32(43):5176-85"}] |