Goat Anti-ITK Antibody

$423.00

Category: Primary Antibodies
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SKU: EB05117
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
MGC126258|MGC126257|tyrosine-protein kinase ITK/TSK|homolog of mouse T-cell itk/tsk|tyrosine-protein kinase LYK|IL2-inducible T-cell kinase|PSCTK2|LYK|EMT|ITK
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and Jurkat cells. Recommended concentration: 10µg/ml.

Flow Cytometry: A customer reported weak staining of Jurkat cells at a concentration:of 10ug/ml.

Accession ID
NP_005537.3
Blocking Peptide
EBP05117
Immunogen
Peptide with sequence C-RLLRQLAEIAESGL, from the C Terminus of the protein sequence according to NP_005537.3.
Peptide Sequence
C-RLLRQLAEIAESGL
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse
Reactive Species
Human
Human Gene ID
3702
Mouse Gene ID
16428
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:2000.
Western Blot
Approx 75kDa band observed in nuclear ysates of cell line Jurkat (calculated MW of 71.8kDa according to NP_005537.3). Recommended concentration: 1-3g/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF

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