Goat Anti-ITK Antibody

$464.00

Category: Primary Antibodies
SKU Unit Size Price

Select a unit size:

SKU: EB08802
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
tyrosine-protein kinase LYK|tyrosine-protein kinase ITK/TSK|homolog of mouse T-cell itk/tsk|PSCTK2|MGC126258|MGC126257|LYK|EMT|IL2-inducible T-cell kinase|ITK
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of Jurkat cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Accession ID
NP_005537.3
Blocking Peptide
EBP08802
Immunogen
Peptide with sequence C-KERPEDRPAFSR, from the internal (near C terminus) of the protein sequence according to NP_005537.3.
Peptide Sequence
C-KERPEDRPAFSR
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Rat, Cow
Reactive Species
Human, Rat
Human Gene ID
3702
Product Grade
IHC Results
Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx 70kDa band observed in Rat Colon, Lung and Spleen lysates (calculated MW of 72.4kDa according to Rat NP_001102295.2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, IF, FC

Related products