Goat Anti-KCNB1 / DRK1 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

voltage-gated potassium channel|potassium voltage-gated channel subfamily B member 1|potassium channel protein DRK1|h-DRK1 K channel|delayed rectifier potassium channel Kv2.1|h-DRK1|KV2.1|DRK1|potassium voltage-gated channel, Shab-related subfamily, member 1|KCNB1

NP_004966.1

EBP08061

Peptide with sequence C-HQYIDADTDDEGQ, from the internal region of the protein sequence according to NP_004966.1.

C-HQYIDADTDDEGQ

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Dog, Cow, Pig

3745

16500

25736

Antibody detection limit dilution 1:4000.

Preliminary experiments gave a 100kDa band and additional stronger bands at approx 50kDa and 38kDa in Human Brain (Cerebellum) and Heart lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the extra bands we observe given the calculated size of 95.9kDa according to NP_004966.1. Both detected extra bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA