Goat Anti-KCNN4 / KCa3.1 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

KCNN4|SK4|putative erythrocyte intermediate|potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4|KCA4|KCa3.1|intermediate conductance calcium-activated potassium channel protein 1|IKCA1|IK1|hSK4|hKCa4|hIKCa1

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/membranes of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

NP_002241.1

EBP09785

Peptide with sequence C-ERQAVNATGHLSD, from the internal region of the protein sequence according to NP_002241.1.

C-ERQAVNATGHLSD

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog

Human, Mouse

3783

16534

65206

Paraffin embedded Human Adrenal Gland, Kidney and Liver. Recommended concentration: 3.75µg/ml.

Antibody detection limit dilution 1:16000.

Approx 50kDa band observed in Human Cerebellum lysates and in lysates of cell line NIH3T3 (calculated MW of 47.7kDa, according to Human NP_002241.1 and Mouse NP_032459.3). An additional band of unknown identity was also consistently observed at 35kDa in Cerebellum. This band was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Adrenal Gland lysate.

Pep-ELISA, WB, IF, FC, IHC