Goat Anti-LARGE (N-terminus) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

LARGE|MDC1D|like-glycosyltransferase|like-acetylglucosaminyltransferase|KIAA0609|acetylglucosaminyltransferase-like protein|acetylglucosaminyltransferase-like 1A

NP_004728.1

EBP09960

Peptide with sequence RYTASSQRERESLE-C, from the N Terminus (near) of the protein sequence according to NP_004728.1.

Reported variants represent identical protein: NP_004728.1, NP_598397.1

RYTASSQRERESLE-C

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Cow

9215

Antibody detection limit dilution 1:32000.

Preliminary experiments gave an approx 125kDa band in Human Kidney, Pancreas and Placenta lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 88.1kDa according to NP_004728.1. The 125kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA