Goat Anti-LEF1 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB07196
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
lymphoid enhancer binding factor-1|TCF1ALPHA|DKFZp586H0919|HGNC:6551|lymphoid enhancer-binding factor 1|LEF1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei of Jurkat and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Accession ID
NP_057353.1; NP_001124185.1; NP_001124186.1; NP_001159591.1
Blocking Peptide
EBP07196
Immunogen
Peptide with sequence C-QHEQRKEQEPKRPH, from the internal region of the protein sequence according to NP_057353.1; NP_001124185.1; NP_001124186.1; NP_001159591.1.
Peptide Sequence
C-QHEQRKEQEPKRPH
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human
Human Gene ID
51176
Mouse Gene ID
16842
Rat Gene ID
161452
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 50--55kDa band observed in nuclear lysates of cell line K562 (calculated MW of 44.2kDa according to NP_057353.1)/ This molecular weight is routinely observed by other sources and was successfully blocked by incubation with the immunising peptide . Recommended concentration: 1-3µg/ml. . Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC

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