Goat Anti-LIGHT / CD258 Antibody

$423.00

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SKU: EB09588
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
TNFSF14|tumor necrosis factor (ligand) superfamily, member 14|CD258|HVEML|LIGHT|LTg|TR2|delta transmembrane LIGHT|herpesvirus entry mediator A|ligand for herpesvirus entry mediator|tumor necrosis factor ligand superfamily, member 14|tumor necrosis factor receptor-like 2|tumor necrosis factor superfamily member LIGHT
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membrane of HeLa cells. Recommended concentration: 10µg/ml.
Accession ID
NP_003798.2; NP_742011.1
Blocking Peptide
EBP09588
Immunogen
Peptide with sequence C-HEVNPAAHLTGANS, from the internal region of the protein sequence according to NP_003798.2; NP_742011.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_003798.2; NP_742011.1).
Peptide Sequence
C-HEVNPAAHLTGANS
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
8740
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx 20-22kDa band observed in Human Cerebellum and Frontal Cortex Brain lysates (calculated MW of 22.4kDa according to NP_742011.1) Recommended concentration: 0.03-0.1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF